Donors were 19-35 "EVEN WITH OUR TRUNCATED AGE RANGE, WE WERE ABLE TO CONFIRM AN INCREASED RISK FOR XX18 ANEUPLOIDY WITH INCREASING DONOR AGE"

Environmental and Molecular Mutagenesis
Volume 30, Issue 2, 1997, Pages 175-183

This article has been cited 62 times in Scopus:
(Showing the 2 most recent)
Eichenlaub-Ritter, U. , Adler, I.-D. , Carere, A.
Gender differences in germ-cell mutagenesis and genetic risk
(2007) Environmental Research

Pacchierotti, F. , Adler, I.-D. , Eichenlaub-Ritter, U.
Gender effects on the incidence of aneuploidy in mammalian germ cells
(2007) Environmental Researc



I: 10.1002/(SICI)1098-2280(1997)30:2<175::AID-EM10>3.0.CO;2-A
Document Type: Article



Use of fluorescence in situ hybridization (FISH] to assess effects of smoking, caffeine, and alcohol on aneuploidy load in sperm of healthy men

Robbins, W.A.a f , Vine, M.F.b , Young Truong, K.c , Everson, R.B.d e

a School of Public Health, Univ. of N. Carolina at Chapel Hill, Chapel Hill, NC, United States
b Epidemiology Department, School of Public Health, Univ. of N. Carolina at Chapel Hill, Chapel Hill, NC, United States
c Biostatistics Department, School of Public Health, Univ. of N. Carolina at Chapel Hill, Chapel Hill, NC, United States
d Human Studies Division, Natl. Hlth. Environ. Effects Res. L., US Environmental Protection Agency, Research Triangle Park, NC, United States
e Karmanos Cancer Inst.-ML Prentis C., Detroit, MI, United States
f Rosenau Hall, CB#7400, School of Public Health, Univ. of N. Carolina at Chapel Hill, Chapel Hill, NC 27599-7400, United States


Abstract

Aneuploidy is a common cause of poor reproductive outcomes in humans and is associated with severe medical problems in liveborn offspring, yet little is known about its underlying cause. A substantial amount of aneuploidy is known to be contributed by the father through cytogenetically abnormal sperm. The purpose of this cross-sectional, observational study was to investigate the potential contribution of common lifestyle exposures (smoking, caffeine, and alcohol) to the aneuploidy load in sperm from 45 healthy male volunteers 19-35 years of age. Sperm FISH (fluorescence in situ hybridization) was used to determine aneuploidy and diploidy frequencies for chromosomes X, Y and 18 across varying exposure levels of smoking, caffeine, and alcohol. Caffeine was significantly associated with increased frequencies of sperm aneuploidy XX18 and XY18, diploidy XY18-18 and the duplication phenotype YY18-18 controlling for alcohol, smoking and donor age. Alcohol was significantly associated with increased frequencies of sperm aneuploidy XX18, diploidy XY18-18 and the duplication phenotype XX18-18 controlling for caffeine, smoking and donor age. There was a suggestive, but not unstable, association between smoking and XX18. Even within our truncated age range, we were able to confirm an increased risk for XX18 aneuploidy with increasing donor age. Sperm FISH proved to be a useful biomarker to detect and compare numerical cytogenetic abnormalities in human sperm cells across differing levels of exposure to smoking, caffeine, and alcohol.

Author Keywords

Aneuploidy; Confounding: smoking, caffeine, alcohol; Fluorescence in situ hybridization; Human sperm and reproduction; Semen studies

Robbins, W.A.; School of Public Health, Univ. North Carolina at Chapel Hill, Chapel Hill, NC 27599-7400, United States;
© Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.